Human SPHK1/SPHK ORF/cDNA clone-Lentivirus plasmid (NM_021972)

Cat. No.: pGMLP000419
Size: 10 µg
Concentration: generally 0.5ug/ul, usually not less than 0.3ug/ul
Leading Time: 3-7 working days

Pre-made Human SPHK1/SPHK Lentiviral expression plasmid for SPHK1 lentivirus packaging, SPHK1 lentivirus production, overexpression stable cell line development, cell transient transfection and gene delivery targeting T/B/NK cells, macrophages, cardiomyocytes, hepatocytes, and neurons.

Our GM-Lentiviral vector is seamlessly integrated with the GM lentivirus packaging system. Discover more about the GM lentivirus packaging system.

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Product Description

Catalog ID pGMLP000419
Gene Name SPHK1
Accession Number NM_021972
Gene ID 8877
Species Human
Product Type Lentivirus plasmid (overexpression)
Insert Length 1197 bp
Gene Alias SPHK
Fluorescent Reporter ZsGreen
Mammalian Cell Selection Puromyocin
Fusion Tag 3xflag (C-Terminal)
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGGATCCAGTGGTCGGTTGCGGACGTGGCCTCTTTGGTTTTGTTTTCTCAGCGGGCGGCCCCCGGGGCGTGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCGGCGGCAAGGGCAAGGCCTTGCAGCTCTTCCGGAGTCACGTGCAGCCCCTTTTGGCTGAGGCTGAAATCTCCTTCACGCTGATGCTCACTGAGCGGCGGAACCACGCGCGGGAGCTGGTGCGGTCGGAGGAGCTGGGCCGCTGGGACGCTCTGGTGGTCATGTCTGGAGACGGGCTGATGCACGAGGTGGTGAACGGGCTCATGGAGCGGCCTGACTGGGAGACCGCCATCCAGAAGCCCCTGTGTAGCCTCCCAGCAGGCTCTGGCAACGCGCTGGCAGCTTCCTTGAACCATTATGCTGGCTATGAGCAGGTCACCAATGAAGACCTCCTGACCAACTGCACGCTATTGCTGTGCCGCCGGCTGCTGTCACCCATGAACCTGCTGTCTCTGCACACGGCTTCGGGGCTGCGCCTCTTCTCTGTGCTCAGCCTGGCCTGGGGCTTCATTGCTGATGTGGACCTAGAGAGTGAGAAGTATCGGCGTCTGGGGGAGATGCGCTTCACTCTGGGCACCTTCCTGCGTCTGGCAGCCCTGCGCACCTACCGCGGCCGACTGGCCTACCTCCCTGTAGGAAGAGTGGGTTCCAAGACACCTGCCTCCCCCGTTGTGGTCCAGCAGGGCCCGGTAGATGCACACCTTGTGCCACTGGAGGAGCCAGTGCCCTCTCACTGGACAGTGGTGCCCGACGAGGACTTTGTGCTAGTCCTGGCACTGCTGCACTCGCACCTGGGCAGTGAGATGTTTGCTGCACCCATGGGCCGCTGTGCAGCTGGCGTCATGCATCTGTTCTACGTGCGGGCGGGAGTGTCTCGTGCCATGCTGCTGCGCCTCTTCCTGGCCATGGAGAAGGGCAGGCATATGGAGTATGAATGCCCCTACTTGGTATATGTGCCCGTGGTCGCCTTCCGCTTGGAGCCCAAGGATGGGAAAGGTGTGTTTGCAGTGGATGGGGAATTGATGGTTAGCGAGGCCGTGCAGGGCCAGGTGCACCCAAACTACTTCTGGATGGTCAGCGGTTGCGTGGAGCCCCCGCCCAGCTGGAAGCCCCAGCAGATGCCACCGCCAGAAGAGCCCTTATGA
ORF Protein Sequence MDPVVGCGRGLFGFVFSAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-T86014-Ab Anti-SPHK1/ SPHK monoclonal antibody
    Target Antigen GM-Tg-g-T86014-Ag SPHK1 VLP (virus-like particle)
    ORF Viral Vector pGMLP000419 Human SPHK1 Lentivirus plasmid
    ORF Viral Vector pGMLP005387 Human SPHK1 Lentivirus plasmid
    ORF Viral Vector pGMLV000633 Human SPHK1 Lentivirus plasmid
    ORF Viral Vector pGMLV001065 Human SPHK1 Lentivirus plasmid
    ORF Viral Vector pGMAAV001003 Human SPHK1 Adeno-associate virus(AAV) plasmid
    ORF Viral Vector vGMLP000419 Human SPHK1 Lentivirus particle
    ORF Viral Vector vGMLP005387 Human SPHK1 Lentivirus particle
    ORF Viral Vector vGMLV000633 Human SPHK1 Lentivirus particle
    ORF Viral Vector vGMLV001065 Human SPHK1 Lentivirus particle
    ORF Viral Vector vGMAAV001003 Human SPHK1 Adeno-associate virus(AAV) particle


    Target information

    Target ID GM-T86014
    Target Name SPHK1
    Gene Group Identifier
    （Target Gene ID in Homo species)         		8877
    Gene ID 100058875 (Equus caballus), 101084918 (Felis catus), 170897 (Rattus norvegicus), 20698 (Mus musculus)
    483329 (Canis lupus familiaris), 618605 (Bos taurus), 710441 (Macaca mulatta), 8877 (Homo sapiens)
    Gene Symbols & Synonyms SPHK1,Sphk1,Sk1,Spk1,1110006G24Rik,SPHK
    Target Alternative Names 1110006G24Rik,Acetyltransferase SPHK1,SK 1,SPHK,SPHK1,SPK 1,Sk1,Sphingosine kinase 1,Sphk1,Spk1
    Uniprot Accession Q8CI15,Q91V26,Q9NYA1
    Additional SwissProt Accessions: Q91V26,Q8CI15,Q9NYA1
    Uniprot Entry Name
    Protein Sub-location Transmembrane Protein
    Category Therapeutics Target
    Disease cancer
    Disease from KEGG Metabolic pathways, Calcium signaling pathway, Sphingolipid signaling pathway, Phospholipase D signaling pathway, Tuberculosis, Sphingolipid metabolism
    Gene Ensembl ENSECAG00000023642, ENSMUSG00000061878, ENSBTAG00000008507, ENSMMUG00000018011, ENSG00000176170
    Target Classification Tumor-associated antigen (TAA)


    About GMVC

    GDU

    GMVC (GM Vector Core) is GeneMedi’s unique platform for QbD Viral vectors Processes development and manufacturing. In GMVC, our core expertise lies in the tailored production of viral vectors, including adeno-associated virus (AAV), lentivirus, and adenovirus. Our state-of-the-art facilities are equipped for scalable manufacturing, ensuring high-quality viral vector production to meet both research and therapeutic needs. Our expert team specializes in process development, leveraging innovative technology and extensive industry knowledge to provide clients with tailored solutions that exceed expectations. GMVC will be the ideal partner for scientists and healthcare professionals seeking reliable and efficient viral vector production services.