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High Efficiency: >90% in most cell lines, including hard-to-transfect cells.
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Low Cytotoxicity: Maintain cell health without media changes.
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Broad Compatibility: For adherent and suspension cells.
OligoMaxi™ RNA Transfection Reagent
High-Efficiency siRNA/miRNA Delivery for Mammalian Cells
Powerful and Versatile Small RNA Delivery
OligoMaxi™ RNA transfection reagent is a powerful siRNA/miRNA transfection reagent designed to ensure highly efficient and reproducible delivery of various small RNAs into mammalian cells. With OligoMaxi™, even at low siRNA concentrations, over 90% transfection efficiency can be achieved in most cell types (e.g., HeLa, MCF7, or NIH-3T3).
For hard-to-transfect suspension cells like K562 and THP-1, OligoMaxi™ still delivers outstanding performance, with transfection efficiency exceeding 80%. Its proprietary formulation ensures minimal cytotoxicity, allowing for healthy cell cultures post-transfection, even without a media change, making it ideal for sensitive experiments and high-throughput screening.
Product Specifications
| Product Name | OligoMaxi™ RNA Transfection Reagent |
| Catalog Series | R-OM Series (R-OM01 to R-OM04) |
| Application | siRNA, miRNA, small RNA transfection |
| Cell Types | Adherent & Suspension Mammalian Cells |
| Transfection Efficiency | >90% in adherent cells, >80% in suspension cells |
| Storage | Store at 4°C. Do not freeze. |
| Shipping | Ships on blue ice / ice packs |
| Shelf Life | 1 Year from date of receipt when stored properly |
Technical Documents
Frequently Asked Questions
What is the siRNA and how do they work?
siRNAs are short, noncoding, double-stranded RNAs that leverage the RNA interference (RNAi) pathway to achieve highly specific gene silencing. They suppress gene expression by targeting and selectively degrading complementary messenger RNA (mRNA), through RNA-induced silencing complex (RISC).
How do I order siRNAs?
You may order siRNAs through our website. The desired target can be searched by Symbol, Gene No, accession Number. Each query will yield three candidates. You may select the desired quantity at that point.
Can I order siRNAs targeted for a specific gene?
You may order siRNAs for the target gene of your choice by entering the gene name or Accession number at our website and selecting one of the listed siRNA candidates. You may also order custom designed siRNA if your model organism is not Human, Mouse or Rat. You can also order siRNAs by sequences, please email us.
What form will my order be in?
For siRNAs, both the sense and antisense strands are synthesized at equimolar concentrations, then annealed and delivered in duplexed, lyophilized form. EPDC-treated water with every order. We recommend reconstituting to 100μM.
I want to conduct an in vitro experiment. What scale should I choose? What purification should I select?
With a 2.5 nmol scale siRNA order, you can transfect ten 24-well plates at 20μM per transfection. For in vitro experiments, the standard small-scale purification method is OPC desalt. For in vivo experiments, HPLC purification is required. For in vivo experiments, HPLC purification is required.
I would like to know how the synthesized siRNA is purified.
To purify newly synthesized siRNA, we have several methods including OPC, HPLC and PAGE. We select a purification method depending on the end-use of the product:
In vitro experiments, OPC purification is enough for use.
in vivo experiments, HPLC is commonly used to reach the desired purity level. We use a reversed-phase resin for HPLC purification, and this will routinely yield up to 90% purity for 21-mer siRNA molecules.